Stem cells are special cells with the ability to make many different types of progeny; embryonic stem cells have an unlimited potential to make all types of cells present in the adult body. The field of regenerative medicine is currently developing cell-based therapies to replace defective cells in our bodies with newly generated cells derived from stem cells. To make a physiologically functioning cell from a stem cell precursor, the cells are typically exposed to genetic, biological, and/or chemical stimulation in an artificial setting. In a normal body, stem cell properties are concomitantly regulated alongside dynamic microenvironment forces (stress, pressure) caused by cell movements within a tissue. Unfortunately, relatively little is known about how these forces actually impact stem cell behavior. With the proposed work, we will develop the tools and techniques needed to characterize stem cell movements within intact mouse embryos by developing novel devices capable of dynamic chemical and mechanical stimulation at precise locations over the developing embryo. The long-term impact of this collaborative engineering and developmental biology endeavor will involve creating new ways of directing stem cells towards therapeutically desirable cell types through biomechanical manipulation. Diminishing the need for genetic or biological manipulation of cells by utilizing mechanical forces could offer both safer and more reliable means of generating transplantable material for cell-based therapies in regenerative medicine.